Synthesis of silver (Ag) nano/micro-particles via green process using Andrographis paniculata leaf extract as a bio-reducing agent
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Abstract
In this work silver nano/micro-particles have been synthesized using sambiloto (Andrographis paniculata) plant extract as a bio-reducing agent. The effects of different plant extract concentrations, AgNO3 precursor concentrations, and reaction time on the synthesized silver nano/micro-particles were investigated. The silver nano/micro-particles samples were then analyzed using UV-Vis spectrophotometer (UV-Vis), X-Ray Diffractometer (XRD), Field Emission Scanning Electron Microscopy (FESEM), Particle Size Analyzer (PSA), and Fourier Transform Infra-Red (FTIR) spectroscopy. The UV-Vis absorbance spectrum of the colloid silver nano/micro-particles exhibited that all samples had absorbance peaks at a wavelength around 450 nm, confirming the formation of silver nano/micro-particles. It was also found that the UV-Vis absorbance peak of the silver nano/micro-particles inversely increased with decreasing AgNO3 solution concentration. Whereas, the higher the sambiloto extract concentration the higher the UV-Vis absorbance peaks. The UV-Vis absorbance peak increased with increasing synthesis time, suggesting that silver nano/micro-particles became more prominent. The UV-Vis absorbance peaks of the silver nano/micro-particles were about 0.0462, 0.0637, 0.0729, and 0.0936 at reaction time of 5, 10, 20, and 40 min, respectively. The XRD analysis result confirmed that the synthesized silver nano/micro-particles were in the form of nanocrystals with a face-centered cubic centered without any impurities. Additionally, the FESEM images showed that the silver nano/micro-particles had the primary particle size of 150-300 nm. There was the formation of some secondary particles with the size of about 0.7-1.5?m due to the agglomeration of primary particles. The particle size distribution analysis further confirmed the presence of primary and secondary particles. Meanwhile, the FTIR analysis confirmed the presence of four main peaks, linked to functional groups in the sambiloto extract and involved in the creation of silver nano/micro-particles.
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References
2. T. Rambaran, R. Schirhagl, Nanotechnology from lab to industry–a look at current trends, Nanoscale Adv. 4 (2022) 3664–3675.
3. S. Malik, K. Muhammad, Y. Waheed, Nanotechnology: A revolution in modern industry, Molecules 28 (2023) 661-687.
4. M. Nasrollahzadeh, S.M. Sajadi, M. Sajjadi, Z. Issaabadi, Applications of nanotechnology in daily life, Interface Sci.Technol. 28 (2019) 113–143.
5. I. Khan, K. Saeed, I. Khan, Nanoparticles: Properties, applications and toxicities, Arab. J. Chem. 12 (2019) 908–931.
6. T. Ohshiro, Nanodevices for biological and medical applications: Development of single-molecule electrical measurement method, Appl. Sci. 12 (2022) 1539-1559.
7. A. Munawar, Y. Ong, R. Schirhagl, M.A. Tahir, W.S. Khan, S.Z. Bajwa, Nanosensors for diagnosis with optical, electric and mechanical transducers, RSC Adv. 9 (2019) 6793–6803.
8. M. Abass Sofi, S. Sunitha, M. Ashaq Sofi, S.K. Khadheer Pasha, D. Choi, An overview of antimicrobial and anticancer potential of silver nanoparticles, J. King Saud Univ. Sci. 34 (2022) 101791.
9. G. Habibullah, J. Viktorova, T. Ruml, Current Strategies for Noble Metal Nanoparticle Synthesis, Nanoscale Res. Lett. 16 (2021) 47-59.
10. S. Medici, M. Peana, V.M. Nurchi, J.I. Lachowicz, G. Crisponi, M.A. Zoroddu, Noble metals in medicine: Latest advances, Coord. Chem. Rev. 284 (2015) 329–350.
11. A. Jouyban, E. Rahimpour, Optical sensors based on silver nanoparticles for determination of pharmaceuticals: An overview of advances in the last decade, Talanta 217 (2020) 121071.
12. S. Dawadi, S. Katuwal, A. Gupta, U. Lamichhane, R. Thapa, S. Jaisi, G. Lamichhane, D.P. Bhattarai, N. Parajuli, Current Research on Silver Nanoparticles: Synthesis, Characterization, and Applications, J Nanomater. 2021 (2021) 6687290.
13. N.P.U. Nguyen, N.T. Dang, L. Doan, T.T.H. Nguyen, Synthesis of silver nanoparticles: from conventional to ‘modern’ methods—a review, Processes 11 (2023) 2617-2634.
14. A. Roy, O. Bulut, S. Some, A.K. Mandal, M.D. Yilmaz, Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity, RSC Adv. 9 (2019) 2673–2702.
15. S. Ahmed, M. Ahmad, B.L. Swami, S. Ikram, A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise, J Adv. Res. 7 (2016) 17–28.
16. R.H. Ahmed, D.E. Mustafa, Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan, Int. Nano Lett. 10 (2020) 1–14.
17. A. Chafidz, A.R. Afandi, B.M. Rosa, J. Suhartono, P. Hidayat, H. Junaedi, Production of silver nanoparticles via green method using banana raja peel extract as a reducing agent, Communications in Science and Technology 5 (2020) 112–118.
18. J.S. Valli, B. Vaseeharan, Biosynthesis of silver nanoparticles by Cissus quadrangularis extracts, Mater. Lett. 82 (2012) 171–173.
19. Hemlata, P.R. Meena, A.P. Singh, K.K. Tejavath, Biosynthesis of Silver Nanoparticles Using Cucumis prophetarum Aqueous Leaf Extract and Their Antibacterial and Antiproliferative Activity Against Cancer Cell Lines, ACS Omega 5 (2020) 5520–5528.
20. R. Mariselvam, A.J.A. Ranjitsingh, A.U.R. Nanthini, K. Kalirajan, C. Padmalatha, P.M. Selvakumar, Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (Family: Arecaceae) for enhanced antibacterial activity, Spectrochim Acta A Mol. Biomol. Spectrosc. 129 (2014) 537–541.
21. Y. Zhang, D. Yang, Y. Kong, X. Wang, O. Pandoli, G. Gao, Synergetic antibacterial effects of silver nanoparticles aloe vera prepared via a green method, Nano Biomed. Eng. 2 (2010) 252–257.
22. A. Bankar, B. Joshi, A.R. Kumar, S. Zinjarde, Banana peel extract mediated novel route for the synthesis of silver nanoparticles, Colloids Surf. A Physicochem. Eng. Asp. 368 (2010) 58–63.
23. T. Prasad, E. Elumalai, Biofabrication of Ag nanoparticles using Moringa oleifera leaf extract and their antimicrobial activity, Asian Pac. J. Trop. Biomed. 1 (2011) 439–442.
24. S. Kaviya, J. Santhanalakshmi, B. Viswanathan, J. Muthumary, K. Srinivasan, Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity, Spectrochim. Acta A. Mol. Biomol. Spectrosc. 79 (2011) 594–598.
25 T. Santhoshkumar, A.A. Rahuman, G. Rajakumar, S. Marimuthu, A. Bagavan, C. Jayaseelan, A.A. Zahir, G. Elango, C. Kamaraj, Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors, Parasitol Res. 108 (2011) 693–702.
26. J.M. Ashraf, M.A. Ansari, H.M. Khan, M.A. Alzohairy, I. Choi, Green synthesis of silver nanoparticles and characterization of their inhibitory effects on AGEs formation using biophysical techniques, Sci. Rep. 6 (2016) 1–10.
27. W.D.P. Rengga, D. Setiawan, Khosiatun, Biosynthesis and kinetics of silver nanoparticles formation by reduction using banana kepok (Musa balbisiana) peel extract, ASEAN J. Chem. Eng. 17 (2017) 77–85.
28. H. Pratikno, P.B. Anggya, F. Fadhila, A. Chafidz, W.D.P. Rengga, Biosynthesis of Silver Nanoparticles Using Banana Raja (Musa paradisiaca var. raja) Peel Extract: Effect of Different Concentrations of the AgNO3 Solution, Key Eng Mater 872 (2021) 61–66.
29. F. Eya’ane Meva, M.L. Segnou, C. Okalla Ebongue, A.A. Ntoumba, P. Belle Ebanda Kedi, V. Deli, M.-A. Etoh, E. Mpondo Mpondo, Spectroscopic synthetic optimizations monitoring of silver nanoparticles formation from Megaphrynium macrostachyum leaf extract, Revista Brasileira de Farmacognosia 26 (2016) 640–646.
30. M. Venkatesham, D. Ayodhya, A. Madhusudhan, A. Santoshi Kumari, G. Veerabhadram, K. Girija Mangatayaru, A Novel Green Synthesis of Silver Nanoparticles Using Gum Karaya: Characterization, Antimicrobial and Catalytic Activity Studies, J Clust. Sci. 25 (2014) 409–422.
31. V. Maragoni, D.-D. Ayodhya, A. Madhusudhan, V. Nagati, V. Guttena, A novel green one-step synthesis of silver nanoparticles using chitosan: Catalytic activity and antimicrobial studies, Appl. Nanosci. 4 (2012) 113-119.
32. T. Ning, Y. Luo, P. Liu, A. Lu, A novel Ag nanoparticles purification method and the conductive ink based on the purified Ag nanoparticles for printed electronics, Journal of Nanoparticle Research 24 (2022) 15-33.
33. A.A. Alshehri, M.A. Malik, Phytomediated Photo-Induced Green Synthesis of Silver Nanoparticles Using Matricaria chamomilla L. and Its Catalytic Activity against Rhodamine B, Biomolecules 10 (2020) 1604-1628.
34. K. Jyoti, M. Baunthiyal, A. Singh, Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics, J Radiat. Res. Appl. Sci. 9 (2016) 217–227.
35. S.S. Royji Albeladi, M.A. Malik, S.A. Al-thabaiti, Facile biofabrication of silver nanoparticles using Salvia officinalis leaf extract and its catalytic activity towards Congo red dye degradation, J. Mater. Res. Technol. 9 (2020) 10031–10044.
36. P. Phanjom, G. Ahmed, Biosynthesis of silver nanoparticles by Aspergillus oryzae (MTCC No. 1846) and its characterizations, Nanosci. Nanotechnol. 5 (2015) 14–21.
37. H. Wang, Y. Jiang, Y. Zhang, Z. Zhang, X. Yang, Md.A. Ali, E.M. Fox, K.S. Gobius, C. Man, Silver nanoparticles: A novel antibacterial agent for control of Cronobacter sakazakii, J. Dairy Sci. 101 (2018) 10775–10791.
38. Bamsaoud, S. F., Basuliman, M. M., Bin-Hameed, E. A., Balakhm, S. M., & Alkalali, A. S. The effect of volume and concentration of AgNO3 aqueous solutions on silver nanoparticles synthesized using Ziziphus Spina–Christi leaf extract and their antibacterial activity. J. Phys. Conf. 1900(1) (2021), 012005.
39. H.J. Noh, A. Im, H.-S. Kim, J.K. Sohng, C.-K. Kim, Y.S. Kim, S. Cho, Y. Park, Antibacterial activity and increased freeze-drying stability of sialyllactose-reduced silver nanoparticles using sucrose and trehalose, J. Nanosci. Nanotechnol. 12 (2012) 3884–3895.
40. N.E.-A. El-Naggar, M.H. Hussein, A.A. El-Sawah, Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotxicity, Sci Rep 7 (2017) 10844-10864.
41. N. Wendri, N.N. Rupiasih, M. Sumadiyasa, Biosintesis nanopartikel perak menggunakan ekstrak daun sambiloto: optimasi proses dan karakterisasi, Jurnal Sains Materi Indonesia 18 (2017) 162–166.