Utilization of extracellular polymeric substances (EPS) immobilized in epoxy polymer as double ion exchanger biosorbent for removal of chromium from aqueous solution

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Lilis Kistriyani
Zainus Salimin
Achmad Chafidz


Cation and industrial pollutant anions are removed from wastewater using organic cation and anion exchange resin. "Extracellular Polymeric Substance" (EPS) from bacterial extraction can accumulate cation and anion elements through biosorption by adsorption mechanism, ion exchange, formation of complex compounds and hydrogen bonds. EPS can be used as an biosorbent and ion exchange bioresin replacing organic resins, because EPS contains organic functional groups that are negatively charged (RCOOH, ROPO3H, ROPO3Na, ROSO3H, ROSO3Na, etc.) cation absorbers and positively charged (ROH, RCNH2HCOOH, etc.) anion absorber. EPS consists of 40-95% polysaccharide compounds, protein 1-60%, nucleic acids 1-10%, lipids 1-10% and the remaining amino acid polymers and other compounds. The tannery industry produces trivalent (Cr+3) chromium pollutants at levels of 15.2 ppm and hexavalent (CrO4-2 or Cr2O7-2) levels of 0.77 ppm which exceeds the standard quality for a total Cr of 0.6 ppm. Cr pollutants are very dangerous for human health. Research had been done on the use of immobilized EPS bioresin in epoxy polymers for chromium binding. EPS was extracted from bacterial activated sludge by centrifugation at 9000 rpm for 20 minutes at 4°C, the filtrate was EPS. The analysis showed EPS content were 16% fat, 12% carbohydrate, and 16% protein. The functional group analysis results with infrared ray spectroscopy (FTIR) showed EPS containing chemical bonds such as -CH, -OH, -NH, and -C=O which proved that EPS extraction contained RCOOH, ROH, and RCNH2HCOOH functional components which were exchanging components cations and anions. Epoxy polymers were prepared by mixing bisphenol A monomers and 1: 1 ratio epichlorohydrin. Immobilized EPS double ion exchange biorecin in epoxy polymers was prepared by mixing 200 mg EPS and 1800 mg epoxy. The binding of chromium ions in the resin was carried out by recirculating the chromium solution through a burette column filled with 2 rams of bioresin at pH 5, 6 and 7. The optimum results gave chromium ion absorption efficiency of 89.20% at pH 5. Column operations could be optimized by varied the amount of bioresin used.


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Kistriyani, L., Salimin, Z., & Chafidz, A. (2020). Utilization of extracellular polymeric substances (EPS) immobilized in epoxy polymer as double ion exchanger biosorbent for removal of chromium from aqueous solution. Communications in Science and Technology, 5(1), 40-44. https://doi.org/10.21924/cst.5.1.2020.179


1. S. Tunali, A. Çabuk, and T. Akar, Removal of lead and copper ions from aqueous solutions by bacterial strain isolated from soil, Chem. Eng. J. 115 (2006) 203–211.
2. M. N. U. Coral, H. Korkmaz, B. Arikan, and G. Coral, Plasmid mediated heavy metal resistances in Enterobacter spp. isolated from Sofulu landfill, in Adana, Turkey, Ann. Microbiol. 55 (2005) 175.
3. S. Özdemir, E. Kilinc, A. Poli, B. Nicolaus, and K. Güven, Biosorption of Cd, Cu, Ni, Mn and Zn from aqueous solutions by thermophilic bacteria, Geobacillus toebii sub.sp. decanicus and Geobacillus thermoleovorans sub.sp. stromboliensis: Equilibrium, kinetic and thermodynamic studies, Chem. Eng. J. 152 (2009) 195–206.
4. D. Long, M. Z. Hashmi, X. Su, and S. Pongpiachan, Cr(VI) reduction by an extracellular polymeric substance (EPS) produced from a strain of Pseudochrobactrum saccharolyticum, 3 Biotechnol. 9 (2019) 111.
5. M. D. G. de Luna, E. D. Flores, M. C. B. Cenia, and M.-C. Lu, Removal of copper ions from aqueous solution by adlai shell (Coix lacryma-jobi L.) adsorbents, Bioresour. Technol. 192 (2015) 841–844.
6. J. Yang et al., Competitive adsorption of heavy metals by extracellular polymeric substances extracted from Klebsiella sp. J1, Bioresour. Technol. 196 (2015) 533–539.
7. J. Feng et al., The adsorption behavior and mechanism investigation of Pb(II) removal by flocculation using microbial flocculant GA1, Bioresour. Technol. 148 (2013) 414–421.
8. J. Wang, Q. Li, M.-M. Li, T.-H. Chen, Y.-F. Zhou, and Z.-B. Yue, Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria, Bioresour. Technol. 163 (2014) 374–376.
9. Y. Tian, Behaviour of bacterial extracellular polymeric substances from activated sludge: a review, Int. J. Environ. Pollut. 32 (2008) 78–89.
10. J. Guo and J. Yu, Sorption characteristics and mechanisms of Pb (II) from aqueous solution by using bioflocculant MBFR10543, Appl. Microbiol. Biotechnol. 98 (2014) 6431–6441.
11. Z. Wang et al., Effect of hexavalent chromium on extracellular polymeric substances of granular sludge from an aerobic granular sequencing batch reactor, Chem. Eng. J. 251 (2014) 165–174.
12. S. Zainus and N. Endang, Biosorption phenomena of chromium, copper, iron and zink by dispersed bacterial extracellular polymeric substance, Int. Nuc. Info. Syst. 47 (2015) 47100094.
13. T. D. Reynolds and P. A. C. Richards, Unit operations and processes in environmental engineering, no. 628.162 R333u Ej. 1. PWS Publishing Company, 1995.
14. G. Tchobanoglus, F. Burton and H. D. Stensel, Wastewater engineering: Treatment and reuse, Am. Water Work. Assoc. J. 95 (2003) 201.
15. K. Nouha, R. S. Kumar and R. D. Tyagi, Heavy metals removal from wastewater using extracellular polymeric substances produced by Cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods, Bioresour. Technol. 212 (2016) 120–129.
16. L. Wang, J. Yang, Z. Chen, X. Liu, and F. Ma, Biosorption of Pb (II) and Zn (II) by extracellular polymeric substance (Eps) of Rhizobium Radiobacter: equilibrium, kinetics and reuse studies, Arch. Environ. Prot. 39 (2013) 129–140.
17. R. Chug, V. S. Gour, S. Mathur, and S. L. Kothari, Optimization of extracellular polymeric substances production using Azotobacter beijreinckii and Bacillus subtilis and its application in chromium (VI) removal, Bioresour. Technol. 214 (2016) 604–608.
18. R. Jin, Y. Liu, G. Liu, T. Tian, S. Qiao, and J. Zhou, Characterization of product and potential mechanism of Cr(VI) reduction by anaerobic activated sludge in a sequencing batch reactor, Sci. Rep. 7 (2017) 1681.
19. R. Dobrowolski, A. Szcze?, M. Czemierska, and A. Jarosz-Wiko?azka, Studies of cadmium(II), lead(II), nickel(II), cobalt(II) and chromium(VI) sorption on extracellular polymeric substances produced by Rhodococcus opacus and Rhodococcus rhodochrous, Bioresour. Technol. 225 (2017) 113.
20. G. Guibaud, S. Comte, F. Bordas, S. Dupuy, and M. Baudu, Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel, Chemosphere 59 (2005) 629–638.
21. S. Zainus and A. A. Pungky, Utilization potency of extracellular polymeric substance as industrials biosorbent and ion exchange resin, Chem. Mat. Eng. 12 (2012) 1-8.
22. E. E. Ergozhin, G. K. Kabulova, A. I. Nikitina, and N. A. Bektenov, Sorption capacity of new cation exchangers based on oil residue and epoxy resin for chromium(III) ions, Russ. J. Appl. Chem. 81 (2008) 1356–1359.
23. Z. Salimin and Wati, Solidification of sludge resulting from biooxidation process of organic waste of phosphate acid purification using epoxy matrix, Proceeding of Scientific Presentation and Meeting, Yogyakarta, June 2008.