Tooth spray with active ingredients casein phosphopeptide – amorphous calcium phosphate (CPP – ACP) and ethanolic extract propolis (EEP) to inhibit tooth demineralization
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Abstract
Dental caries is a disease caused by the microbial infection that produces localized damage to the tissue classification. The process begins by demineralization in hard tissue followed by damage to the organic material. Dental caries happens because of demineralization without balanced by remineralization. In this study, tooth powder spray and tooth liquid spray was made with active ingredients of 10% w/w CPP-ACP (Casein Phosphopeptide - Amorphous Calcium Phosphate) and EEP (Ethanolic Extract Propolis), with the variation of 0%, 2%, and 4% w/w EEP in each preparation to inhibit demineralization. Each preparation should pass organoleptic and viscosity study to maintain the quality. pH of each preparation was evaluated to ensure that each preparation has pH above the critical pH of the oral environment. The stability of polyphenol as the antibacterial agent was evaluated for a month to assure the bioavailability in the preparations. The result shows that each preparation has enough organoleptic aspect and proper viscosity. pH level in each preparation is stable above critical pH of mouth environment (5.5), it ranges between 6.5 – 7.12. Polyphenol content in each preparation is stable with highest polyphenol content in tooth powder spray of 4% EEP, i.e. 1.39 – 1.45% (w/w). Qualitative evaluation of tooth morphology by SEM shows that preparations can inhibit enamel demineralization relatively to negative control.
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Sahlan, M., & Purwanti, L. (2016). Tooth spray with active ingredients casein phosphopeptide – amorphous calcium phosphate (CPP – ACP) and ethanolic extract propolis (EEP) to inhibit tooth demineralization. Communications in Science and Technology, 1(2). https://doi.org/10.21924/cst.1.2.2016.24
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References
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11. Liberio, Silvana A., et al. The Potential Use of Propolis as Cariostatic Agent and Its Actions on Mutans Group Streptococci. (2009), Journal of Ethnopharmacology, pp. 1-9.
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13. Selwitz, Robert H, Ismail, Amid I and Pitts, Nigel B. Dental Caries. (2007), Lancet, pp. 51-59.
14. Farooq, Imran, et al. A Review of Novel Dental Caries Preventive Material : Casein Phosphopeptide - Amorphous Calcium Phosphate (CPP-ACP) Complex. (2013), King Saud University Journal of Dental Sciences, pp. 47-51.
2. Clark, Kristin Dumboski. The Efficacy of 37% Phosphoric Acid + Mi Paste Plus on Remineralization of Enamel White Spot Lesions. Iowa : University of Iowa (2011).
3. Cury, J.A. and Tenuta, L.M. Enamel Remineralization : Controlling The Caries Disease or Treating Early Caries Lessions? (2009), Braz Oral Res, pp. 23-30.
4. Featherstone, J.D.B. Dental Caries : A Dynamic Disease Process. (2008), Australia Dental Journal, pp. 286-291.
5. Pulido, MT, et al. The Inhibitory Effect of MI Paste, Fluoride and Combination of Both on The Progression of Artificial Caries - Like Lesions in Enamel. (2008), Operative Dentistry Journal 33(5), pp. 550-555.
6. Sabir, Ardo. Aktivitas antibakteri flavonoid propolis Trigona sp terhadap bakteri Streptococcus mutans (in vitro). Majalah Kedokteran Gigi. 38(3) (2005), pp. 135-141.
7. Hongal, Sudhir, et al. The Effect of 30% Ethanolic Extract of Indian Propolis on Replica of Human Dentin Compared Against Commmercially Available Desentizing Agent : A Methodological SEM Study In Vitro. (2014), Pharmacognosy Res Apr - Jun 6(2), pp. 113 - 119.
8. Sahlan, Muhamad and Pramadewi, Indrianti. Nanoencapsulation of The Flavaonoids Isolated from Phaleria macrocarpa Leaf by Casein Micelle. (2012), Int J Bio Sci July 3(3), pp. 472 - 478.
9. Reynold, Eric. Casein Phosphopeptide - Amorphous Calcium Phosphate Nano Complexes. Unites States patent 0037948, (2005).
10. Jayarajan, J., et al. Efficacy of CPP-ACP and CPP-ACPF on Enamel Remineralization - An In Vitro Study Using Scanning Electron Microscope and DIAGNOdent. (2011), Indian J Dent Res, pp. 77 - 82.
11. Liberio, Silvana A., et al. The Potential Use of Propolis as Cariostatic Agent and Its Actions on Mutans Group Streptococci. (2009), Journal of Ethnopharmacology, pp. 1-9.
12. Sulistyani, Nindya. Pembuatan Tooth Gel yang Mengandung Casein Phosphopeptide - Amorphous Calcium Phosphate dan Propolis untuk Menghambat Demineralisasi. (2016) University of Indonesia. Bachelor Thesis.
13. Selwitz, Robert H, Ismail, Amid I and Pitts, Nigel B. Dental Caries. (2007), Lancet, pp. 51-59.
14. Farooq, Imran, et al. A Review of Novel Dental Caries Preventive Material : Casein Phosphopeptide - Amorphous Calcium Phosphate (CPP-ACP) Complex. (2013), King Saud University Journal of Dental Sciences, pp. 47-51.