In vitro anthelmintic activity of pineapple peel juice (Ananas comosus (L.) Merr.) against Paramphistomum sp.
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Keywords:
Pineapple Peel Juice, Anthelmintic, Paramphistomum sp
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Abstract
Pineapple peel (Ananas comosus (L.) Merr.) has a potential natural-based anthelmintic agent. This study aimed to determine the effective concentration of pineapple peel juice against Paramphistomum sp. in vitro. Adults Paramphistomum sp. were divided into 5 test groups, the control group contained Albendazole 10% w/v, the nontreatment group contained NaCl 0.9% w/v and the treatment group contained pineapple peel juice with concentration of 12.5, 20, and 25% w/v. The number of live flukes and the motility scores were recorded every 15 minutes for 5 hours of incubation. The Survival Index (SI) and Relative Motility (RM) of flukes were calculated and statically analyzed using SPSS version 23 software. SI values of flukes in the 12.5, 20, and 25% w/v concentration of treatment group were 46.3, 50.0, and 38.9% respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1, 42.0, and 34.1 respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1359, 42.0249, and 34.1174 respectively. This research showed that, pineapple peel juice 25% w/v was effective against Paramphistomum sp. with survival index and relative motility values comparable to Albendazole 10% w/v (p <0.05).
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Damiyati, S. Y., Pratama, I. S., & Tresnani, G. (2021). In vitro anthelmintic activity of pineapple peel juice (Ananas comosus (L.) Merr.) against Paramphistomum sp. Communications in Science and Technology, 6(1), 49-54. https://doi.org/10.21924/cst.6.1.2021.394
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References
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N.M. Armani, N.K.H. Saraswati, I.M.G. Sutawijaya, and Yunanto., Surveilans parasit gastrointestinal pada ternak sapi dan kerbau di Provinsi Bali, NTB dan NTT tahun 2016. Buletin Veteriner, Balai Besar Veteriner Denpasar. (2017) 48-66.
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M.R. Ilha, A.P. Loretti, and A.C. Reis, Wasting and mortality in beef cattle parasitized by Eurytrema coelamaticum in the state of Parana, Southern Brazil, Vet. Parasitol. 133 (2005) 49-60.
M.Q. Bilal, A. Hameed, and T. Ahmad, Prevalence of gastrointestinal parasites in buffalo and cow calves in rural areas of Toba Tek Singh, Pakistan, J. Anim. Plant Sci. 19 (2009) 67-70.
M.K. Khan, M.S. Sajid, M.N. Khan, Z. Iqbal, and M.U. Iqbal, Bovine fasciolosis: Prevalence, effects of treatment on productivity and cost benefit analysis in five districts of Punjab, Pakistan, Res. Vet. Sci. 87 (2009) 70-75.
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S.G. Wynn, and B.J. Fougere. Veterinary herbal medicine. St. Louis: Mosby. 2007.
J.M.Y. Cormanes, H.P. Portugaliza, and M.M. Quilicot, In vivo anthelmintic activity of pineapple (Ananas comosus Merr.) fruit peeling juice in semi-scavenging Philippine native chicken naturally co-infected with Ascaridia galli and Heterakis gallinarum. Livest. Res. Rural. Dev. 28 (2016) 1-12.
S.B. Widodo, Uji aktivitas jus limbah nanas (Ananas comosus (L) Merr) sebagai antelmintik cacing usus pada sapi. Thesis, Universitas Mataram, Mataram, 2019.
D. Lawal, Medicinal, pharmacological, and phytochemical potentials of Ananas comosus Linn. peel - a review. Bajopas. 6 (2013) 101-104.
M.F.A. Al-Amura, Z.A. Hassen, and B.H. Al-Mhanawi, Staining technique for helminth parasites by use red beet (Beta vulgaris L.) extract. Bas. J. Vet. Res. 11 (2012) 283-292.
Y. Watanabe, S. Nishida, K. Zenke, H.K. Hui, N. Itoh, and T. Yoshinaga, Development of the macronucleus of Cryptocaryon irritans, a parasitic ciliate of marine teleosts, and its ingestion and digestion of host cell. Fish Pathol. 52 (2016) 112-120.
H. Mehlhorn. Encyclopedia of parasitology. 3rd ed. New York: Springer. 2008.
N. Lorsuwannarat, et al., 2014. The in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of Fasciola gigantica. Exp. Parasitol. 136 (2014) 5-13.
C.M. Hendrix and E. Robinson. Diagnostic parasitology for veterinary technicians.5th ed. New York: Elsevier. 2017.
K.M. Husson, N.A.M. Oliver, and M.W. Robinson, Paramphistomosis of ruminants: an emerging parasitic disease in Europe. Trends Parasitol. 33 (2017) 836-844.
V. Choudhary, et al., Morphological and histological identification of Paramphistomum cervi (Trematoda: Paramphistoma) in the rumen of infected sheep. Vet. World. 8. (2015) 125-129.
D. Masih, N. Singh, and A. Singh, Red Beetroot: A source of natural colourant and antioxidants: A review. J. Pharmacogn. Phytochem. 8 (2019) 162-166.
M.I. Udonkang, I.J. Inyang, A.N. Ukorebi, F. Effiong, U. Akpan, and I.E. Bassey, spectrophotometry, physiochemical properties, and histological staining potential of aqueous and ethanol extracts of beetroot on various tissues of an albino rat. Biomed. Hub. 3 (2018) 1-10.
J.P. Zryd and C. Laurent, Betalain. In: Davies K. (Eds). Plant pigments and their manipulation. Oxford: Annual Plant Review. 2004.
J.A. Rattenbury. Specific staining of nucleolar substance with aceto-carmine. Stain Technol. 27 (1952) 113-120.
G.J.H. Grubben, and O.A. Denton. Plant resources of Tropical Africa 2. Wageningen: PROTA Foundation/Backhuys Publisher/CTA. 2004
V. Sivakumar, J.L. Anna, Vijayeeswarri, and G. Swaminathan, Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather. Ultrason. Sonochem. 16 (2009) 782-789
M. Yeniocak, O. Goktas, M. Colak, E. Ozen, and M. Ugurle, Natural coloration of wood material by red beetroot (Beta vulgaris) and determination color stability under UV exposure. Technol. 17 (2015) 711-722.
D. Plumb. Plumb's veterinary drug handbook. 7th ed. Stockholm: PharmaVet. Inc. 2011.
S.T. Purnamasari, 2017. Uji aktivitas anthelmintik ekstrak etanol daun jarak pagar (Jatropha curcas Linn) terhadap cacing Paramphistomum sp. secara in vitro. Thesis, Universitas Hasanuddin, Makassar, 2017
E. Hossain, G. Chandra, A.P. Nandy, S.C. Mandal, and J.K. Gupta, Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum. Parasitol. Res. 110 (2012) 1097-1102.
N. Saowakon, N. Lorsuwannarat, N. Changklungmoa, C. Wanichanon, and P. Sobhon, Paramphistomum cervi: The in vitro effect of plumbagin on motility, survival and tegumentt structure. Exp. Parasitol. 133 (2013) 179-186.
J. Adli and S. Sundari, Daya antihelmintik nanas (Ananas comosus) terhadap Ascaris lumbricoides secara in vitro. Jurnal Mutiara Medika. 8 (2008) 107-112.
P. Anuracrpeeda, R. Chawengkirttikul, B. Panyarachun, P. Puttarak, P. Koedrith, and N. Intaratat, The In Vitro Anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica. Parasitol. p. 1-12. 2013.
D. Jacobs, F. Mark, G. Lynda, and H. Carlos, Principle of veterinary parasitology. Oxford: Wiley-Blackwell. 2015.
M. Kwolek-Mirek and R. Zadrag-Tecza, Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Res. 14 (2014) 1068-1079.
H.A. Shalaby, A.H. El Namaky, R.A. Kamel, and A.A Derbala, Tegumenttal surface changes in adult Paramphistomum microbothrium (Fischoeder 1901) following in vitro administration of artemether. J. Helminthol. 84 (2009) 115-122.
H. Hoste, F. Jackson, A. Spiridoula, S.M. Thamsborg, and S.O. Hoskin, The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends Parasitol. 22 (2006) 253-261.
M.G.S. Septiadi, I.M. Dwinata, and I.B.M. Oka, Vermisidal dan ovisidal getah biduri (Calotropis spp.) terhadap Fasciola gigantica secara in vitro. Indonesia Medicus Veterinus. 5 (2016) 54-60.
H. Roy, Preliminary phytochemical investigation and anthelmintic activity of Acanthospermum hispidum DC. Journal of Pharmaceutical Science and Technology. 2 (2010) 217-221.
S. Poolperm and W. Jiraungskoorskul, An update review on the anthelmintic activity of bitter gourd, Momordica charantia. Pharmacogn. Rev. 11 (2017) 31-4.
Whiteland, H.L., et al., An abies procera-derived tetracyclic triterpene containing a steroid-like nucleus core and a lactone side chain attenuates in vitro survival of both Fasciola hepatica and Schistosoma mansoni. Int. J. Parasitol-Drug. 8 (2018) 465-474.
B.P.S. Capece, S. Calsamigliat, G. Castells, M. Arboix, and C. Cristofol, Effect of ruminal microflora on the biotransformation of netobimin, albendazole, albendazole sulfoxide, and albendazole sulfoxide enantiomers in an artificial rumen. J. Anim. Sci. 79 (2001) 1288-1294.
P. Frutos, G. Hervas, F.J. Giraldez, and A.R. Mantecon, Review tanin and ruminant nutrition. Span. J. Agric. Res. (2004) 191-202.
S.Y. Damiyati, I.S. Pratama, and G.Tresnani, Karakterisasi jus kulit nanas (Ananas comosus L. Merr). BioWallacea. 7 (2021) 84-90.
S. Melaku and M. Addis., Prevalence and intensity of paramphistomum in ruminants slaughtered at Debre Zeit Industrial Abattoir, Ethiopia, Glob. Vet. 8 (2012) 315–319.
T.R.F. Njoku, and B.E.B. Nwoko, Prevalence of paramphistomiasis among sheep slaughtered in some selected abattoirs in Imo State, Nigeria, Science World J. 4 (2009) 12-15.
N. Ozdal, A. Gul, F. Ilhan, and S. Deger, Prevalence of paramphistomum infection in cattle and sheep in Van Province, Turkey, Helminthologia. 47 (2010) 20-24.
N.M. Armani, N.K.H. Saraswati, I.M.G. Sutawijaya, and Yunanto., Surveilans parasit gastrointestinal pada ternak sapi dan kerbau di Provinsi Bali, NTB dan NTT tahun 2016. Buletin Veteriner, Balai Besar Veteriner Denpasar. (2017) 48-66.
E. Murtiyeni, Juarini, dan J. Manurung. Penyakit parasit pada ternak ruminansia. Bogor: Balai Penelitian Veteriner, 2009.
M.R. Ilha, A.P. Loretti, and A.C. Reis, Wasting and mortality in beef cattle parasitized by Eurytrema coelamaticum in the state of Parana, Southern Brazil, Vet. Parasitol. 133 (2005) 49-60.
M.Q. Bilal, A. Hameed, and T. Ahmad, Prevalence of gastrointestinal parasites in buffalo and cow calves in rural areas of Toba Tek Singh, Pakistan, J. Anim. Plant Sci. 19 (2009) 67-70.
M.K. Khan, M.S. Sajid, M.N. Khan, Z. Iqbal, and M.U. Iqbal, Bovine fasciolosis: Prevalence, effects of treatment on productivity and cost benefit analysis in five districts of Punjab, Pakistan, Res. Vet. Sci. 87 (2009) 70-75.
J.U. Khan, T. Akhtar, A. Maqbool, and A. Anees, Epidemiology of paramphistomiasis in buffaloes under different managemental conditions at four districts of Punjab Province, Pakistan, Iran. J. Vet. Res. 7 (2006) 68-72.
V. Tandon, B. Roy, J.A. Shylla, and S. Ghatani. Amphistomes. In: Toledo, R., dan Fried, B. (Eds.). Digenetic trematodes. New York: Springer. 2014.
M.A. Taylor, R.L. Coop, and R.L. Wall. Veterinary parasitology. 4th ed. Oxford: Wiley Blackwell. 2016.
T.W. Sardjono. Helmintologi kedokteran dan veteriner. Malang: Universitas Brawijaya Press. 2017.
F. Kristiyani, N. Aini, and Wijayanti A.D., Evaluasi pengobatan trematodiasis menggunakan albendazol pada sapi di Kecamatan Pakem, Sleman, Daerah Istimewa Yogyakarta, Jurnal Sain Veteriner. 37 (2019) 104-111.
M.H. Babu, J.K. Rao, and T.V.V.S.R Reddi. Ethnoveterinary medicine of Eastern Ghats Ajacent Deccan Region. In: Pullaiah, T., Krishnamurty, K.V., dan Bahadur, B. (Eds.). Ethnobotany of India Volume 1. Oakville: Apple Academic Press Inc. 2017.
Z. Iqbal, S.M Akhtar, Z. Sindhu, M.N. Khan, and A. Jabbar, Herbal deflukeers in livestock - a traditional therapy. Int. J. Agric. Biol. 5 (2003) 1-7.
R. Kumarasingha, S. Preston, T-C Yeo, D.S.L. Lim, C-L. Tu, E.A. Palombo, J.M. Shaw, R.B. Gasser, and P.R. Boag, Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus. Parasit. Vectors. 9 (2016) 199-206.
S.G. Wynn, and B.J. Fougere. Veterinary herbal medicine. St. Louis: Mosby. 2007.
J.M.Y. Cormanes, H.P. Portugaliza, and M.M. Quilicot, In vivo anthelmintic activity of pineapple (Ananas comosus Merr.) fruit peeling juice in semi-scavenging Philippine native chicken naturally co-infected with Ascaridia galli and Heterakis gallinarum. Livest. Res. Rural. Dev. 28 (2016) 1-12.
S.B. Widodo, Uji aktivitas jus limbah nanas (Ananas comosus (L) Merr) sebagai antelmintik cacing usus pada sapi. Thesis, Universitas Mataram, Mataram, 2019.
D. Lawal, Medicinal, pharmacological, and phytochemical potentials of Ananas comosus Linn. peel - a review. Bajopas. 6 (2013) 101-104.
M.F.A. Al-Amura, Z.A. Hassen, and B.H. Al-Mhanawi, Staining technique for helminth parasites by use red beet (Beta vulgaris L.) extract. Bas. J. Vet. Res. 11 (2012) 283-292.
Y. Watanabe, S. Nishida, K. Zenke, H.K. Hui, N. Itoh, and T. Yoshinaga, Development of the macronucleus of Cryptocaryon irritans, a parasitic ciliate of marine teleosts, and its ingestion and digestion of host cell. Fish Pathol. 52 (2016) 112-120.
H. Mehlhorn. Encyclopedia of parasitology. 3rd ed. New York: Springer. 2008.
N. Lorsuwannarat, et al., 2014. The in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of Fasciola gigantica. Exp. Parasitol. 136 (2014) 5-13.
C.M. Hendrix and E. Robinson. Diagnostic parasitology for veterinary technicians.5th ed. New York: Elsevier. 2017.
K.M. Husson, N.A.M. Oliver, and M.W. Robinson, Paramphistomosis of ruminants: an emerging parasitic disease in Europe. Trends Parasitol. 33 (2017) 836-844.
V. Choudhary, et al., Morphological and histological identification of Paramphistomum cervi (Trematoda: Paramphistoma) in the rumen of infected sheep. Vet. World. 8. (2015) 125-129.
D. Masih, N. Singh, and A. Singh, Red Beetroot: A source of natural colourant and antioxidants: A review. J. Pharmacogn. Phytochem. 8 (2019) 162-166.
M.I. Udonkang, I.J. Inyang, A.N. Ukorebi, F. Effiong, U. Akpan, and I.E. Bassey, spectrophotometry, physiochemical properties, and histological staining potential of aqueous and ethanol extracts of beetroot on various tissues of an albino rat. Biomed. Hub. 3 (2018) 1-10.
J.P. Zryd and C. Laurent, Betalain. In: Davies K. (Eds). Plant pigments and their manipulation. Oxford: Annual Plant Review. 2004.
J.A. Rattenbury. Specific staining of nucleolar substance with aceto-carmine. Stain Technol. 27 (1952) 113-120.
G.J.H. Grubben, and O.A. Denton. Plant resources of Tropical Africa 2. Wageningen: PROTA Foundation/Backhuys Publisher/CTA. 2004
V. Sivakumar, J.L. Anna, Vijayeeswarri, and G. Swaminathan, Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather. Ultrason. Sonochem. 16 (2009) 782-789
M. Yeniocak, O. Goktas, M. Colak, E. Ozen, and M. Ugurle, Natural coloration of wood material by red beetroot (Beta vulgaris) and determination color stability under UV exposure. Technol. 17 (2015) 711-722.
D. Plumb. Plumb's veterinary drug handbook. 7th ed. Stockholm: PharmaVet. Inc. 2011.
S.T. Purnamasari, 2017. Uji aktivitas anthelmintik ekstrak etanol daun jarak pagar (Jatropha curcas Linn) terhadap cacing Paramphistomum sp. secara in vitro. Thesis, Universitas Hasanuddin, Makassar, 2017
E. Hossain, G. Chandra, A.P. Nandy, S.C. Mandal, and J.K. Gupta, Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum. Parasitol. Res. 110 (2012) 1097-1102.
N. Saowakon, N. Lorsuwannarat, N. Changklungmoa, C. Wanichanon, and P. Sobhon, Paramphistomum cervi: The in vitro effect of plumbagin on motility, survival and tegumentt structure. Exp. Parasitol. 133 (2013) 179-186.
J. Adli and S. Sundari, Daya antihelmintik nanas (Ananas comosus) terhadap Ascaris lumbricoides secara in vitro. Jurnal Mutiara Medika. 8 (2008) 107-112.
P. Anuracrpeeda, R. Chawengkirttikul, B. Panyarachun, P. Puttarak, P. Koedrith, and N. Intaratat, The In Vitro Anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica. Parasitol. p. 1-12. 2013.
D. Jacobs, F. Mark, G. Lynda, and H. Carlos, Principle of veterinary parasitology. Oxford: Wiley-Blackwell. 2015.
M. Kwolek-Mirek and R. Zadrag-Tecza, Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Res. 14 (2014) 1068-1079.
H.A. Shalaby, A.H. El Namaky, R.A. Kamel, and A.A Derbala, Tegumenttal surface changes in adult Paramphistomum microbothrium (Fischoeder 1901) following in vitro administration of artemether. J. Helminthol. 84 (2009) 115-122.
H. Hoste, F. Jackson, A. Spiridoula, S.M. Thamsborg, and S.O. Hoskin, The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends Parasitol. 22 (2006) 253-261.
M.G.S. Septiadi, I.M. Dwinata, and I.B.M. Oka, Vermisidal dan ovisidal getah biduri (Calotropis spp.) terhadap Fasciola gigantica secara in vitro. Indonesia Medicus Veterinus. 5 (2016) 54-60.
H. Roy, Preliminary phytochemical investigation and anthelmintic activity of Acanthospermum hispidum DC. Journal of Pharmaceutical Science and Technology. 2 (2010) 217-221.
S. Poolperm and W. Jiraungskoorskul, An update review on the anthelmintic activity of bitter gourd, Momordica charantia. Pharmacogn. Rev. 11 (2017) 31-4.
Whiteland, H.L., et al., An abies procera-derived tetracyclic triterpene containing a steroid-like nucleus core and a lactone side chain attenuates in vitro survival of both Fasciola hepatica and Schistosoma mansoni. Int. J. Parasitol-Drug. 8 (2018) 465-474.
B.P.S. Capece, S. Calsamigliat, G. Castells, M. Arboix, and C. Cristofol, Effect of ruminal microflora on the biotransformation of netobimin, albendazole, albendazole sulfoxide, and albendazole sulfoxide enantiomers in an artificial rumen. J. Anim. Sci. 79 (2001) 1288-1294.
P. Frutos, G. Hervas, F.J. Giraldez, and A.R. Mantecon, Review tanin and ruminant nutrition. Span. J. Agric. Res. (2004) 191-202.
S.Y. Damiyati, I.S. Pratama, and G.Tresnani, Karakterisasi jus kulit nanas (Ananas comosus L. Merr). BioWallacea. 7 (2021) 84-90.